Vitamins Vitamin A Vitamin B Vitamin C ○  Vitamin C - Plasma ○  Post IVC Vitamin D Vitamin E Beta Carotene Lutein Lycopene Folic Acid Profiles

Vitamin C - Plasma Turn-around time = 5 to 7 days. Specimen collection, preparation and transport is of vital importance for accurate results. Collect the specimen in a cold heparin or EDTA tube. Place tube in ice water and centrifuge immediately. Place 3.0 mL of plasma in 4.5 mL of cold 3% metaphosphoric acid (MPA, supplied with kit). Mix vigorously and freeze. Ship frozen in the MPA tube. Vitamin C is also called ascorbic acid. There have been over 7000 articles written about vitamin C. It is a strong water-soluble antioxidant. Vitamin C stimulates the immune system in seven different ways and is important in collagen formation. It is important in the synthesis of connective tissue, and in wound healing. It helps regulate lipids in blood, iron absorption and is important in synthesis of hormones in stress situations. It helps regenerate vitamin E in the body. It is found in many fruits and vegetables such as sweet peppers, broccoli, cauliflower, lemons, strawberries, asparagus, spinach, oranges, cantaloupe, grapefruit and tomatoes. Deficiency of vitamin C results in the classical disease of scurvy. Other conditions are pain in joints, slow wound healing, lower disease resistance, easy bruising, bleeding gums, loose teeth, irritability, premature wrinkling and aging of skin. Like most other vitamins, humans cannot make vitamin C. The RDA for vitamin C is 75 mgs for females, 90 mgs for males. A suggested supplemental dose is 100 to 2000 mg. The methodology is by HPLC. The normal value established in our laboratory is 0.6 to 2.0 mg/dL. ▲ TOP Vitamin C - Plasma, Post IVC Turn-around time = 5 to 7 days. Specimen type and preparation is the same as for plasma vitamin C. Additional information required on the requisition is the grams of vitamin C given (15 grams, 25 grams, 50 grams, etc). Draw specimen from vein on opposite arm used for IVC. Process specimen as above for vitamin C. Although controversial, high dose intravenous vitamin C (ascorbic acid) has been used to treat various conditions. These include various viral infections and cancer. A protocol for use of high dose vitamin C may be obtained by writing, The Center for the Improvement of Human Functioning, International, Inc., 3100 N. Hillside, Wichita, KS 67219. Normal values: Normal ranges do not apply. The value obtained depends on the amount of vitamin C given IV and the disease or physical status of the patient. Selected references: Padayatta SJ, He S, et al. Vitamin C pharmacokinetics: Implications for oral and intravenous use. Ann Intern Med. 2004;140:535-537. Riordan NH, Riordan HD, Jackson JA, et al. Intravenous ascorbate as a tumor cytotoxic chemotherapeutic agent. Med Hypothesis. 1995; 44:207-13. Riordan NH, Riordan HD, Casciari JP, Jackson JA. Clinical and experimental experiences with intravenous vitamin C. 2000; J Orthomol Med. 15:201-213. Cameron E, Pauling L. Cancer and vitamin C. Philadelphia: Camino Books;1993. Jackson JA, Riordan HD, Hunninghake RE, et al. High dose intravenous vitamin C and long-time survival of a patient with cancer of head of the pancreas. 1995; J Orthomol Med. 1995; 10:87. Washko PW, Hartzell WO, Levine M. Ascorbic acid analysis using HPLC with coulometric electrical chemical detection. Analytical Biochemistry. 1989; 181: 276-282. ▲ TOP Back to Vitamin Home Page

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